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Last updated: 2017/12/10

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DNASE1 (Bovine) Recombinant ProteinBioActive

  • Catalog # : P5311
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  • Specification
  • Product Description:
  • Lyophilized. Animal derived components free. RNases & proteases free. Recombinant protein expressed in Pichia pastoris.
  • Host:
  • Yeast
  • Theoretical MW (kDa):
  • 31.3
  • Form:
  • Lyophilized
  • Preparation Method:
  • Yeast expression system
  • Activity:
  • >=5000 units per mg protein. One Unit causes an increase in absorbance at 260 nm of 0.001 per minute per ml at 25°C when acting upon highly polymerized DNA at pH 5.0.
  • Recommend Usage:
  • Deoxyribonuclease I Assay; Removing genomic DNA from RNA preparations prior to RT-PCR; Degradation of DNA templates after transcription reactions; Removing unwanted DNA from samples prior to Northern blotting; Removing DNA during biopharma and bioprocessing procedures.
  • Storage Instruction:
  • Store at 4°C on dry atmosphere.
  • Note:
  • Contains glycine as a stabilizer. All equipment used to handle product should be RNase-free. Glassware and spatulas need to be heat-treated.
  • Biological function:
  • Bovine pancreatic deoxyribonuclease is an endonuclease which splits phosphodiester linkages, preferentially adjacent to a pyrimidine nucleotide yielding polynucleotides with free hydroxyl group at the 3' position and phosphate group at the 5' position.
  • Applications
  • Enzyme Activity
  • Application Image
  • Enzyme Activity
  • Gene Information
  • Gene Name:
  • DNASE1
  • Gene Alias:
  • -
  • Gene Description:
  • deoxyribonuclease I
  • Other Designations:
  • -
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