Solution. Animal derived components free. RNases & proteases free. Recombinant protein solution expressed in Pichia pastoris.
Theoretical MW (kDa):
Yeast expression system
>=2 units per ml. One Unit causes an increase in absorbance at 260 nm of 0.001 per minute per ml at 25°C when acting upon highly polymerized DNA at pH 5.0.
Deoxyribonuclease I Assay; Removing genomic DNA from RNA preparations prior to RT-PCR; Degradation of DNA templates after transcription reactions; Removing unwanted DNA from samples prior to Northern blotting; Removing DNA during biopharma and bioprocessing procedures.
10x reaction buffer
In 5 mM Calcium Acetate, 4 mg/ml glycine (stabilizer), pH 5.0 and 50% glycerol
Store at -20°C.
Bovine pancreatic deoxyribonuclease is an endonuclease which splits phosphodiester linkages, preferentially adjacent to a pyrimidine nucleotide yielding polynucleotides with free hydroxyl group at the 3' position and phosphate group at the 5' position.