Deoxyribonuclease I
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Specification
Product Description
Lyophilized. Contains ? 0.0005% RNase.
Biological function
Bovine pancreatic deoxyribonuclease is an endonuclease which splits phosphodiester linkages, preferentially adjacent to a pyrimidine nucleotide yielding polynucleotides with free hydroxyl group at the 3' position and phosphate group at the 5' position.
Host
Bovine
Theoretical MW (kDa)
31.3
Form
Lyophilized
Preparation Method
Native protein chromatographically purified from Bovine Pancreas
Activity
>=2,000 Kunitz units per mg dry weight. One Unit causes an increase in absorbance at 260 nm of 0.001 per minute per ml at 25°C when acting upon highly polymerized DNA at pH 5.0.
Recommend Usage
Deoxyribonuclease I Assay; Nick translation; DNA mapping; Isolation of nuclear RNA and protein; Plasmid construction; RNA polymerase synthesis of RNA probes and RT-PCR.
Storage Instruction
Store at 4°C on dry atmosphere.
Note
Contains glycine as a stabilizer. All equipment used to handle product should be RNase-free. Glassware and spatulas need to be heat-treated.
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Applications
Enzyme Activity
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Gene Info — DNASE1
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