Alkaline Phosphatase (E. coli)
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Specification
Product Description
Solution. Purified. Ribonuclease ≤ 0.002% by weight as RNase A using a poly C assay. Phosphodiesterase not detectable when assayed at 0.1 mg/ml with bis(p-nitrophenyl) phosphate.
Biological function
Alkaline phosphatase is a broad term associated with non-specific phosphomonoesterases with an alkaline pH optimum.
Host
Escherichia coli
Form
Liquid
Preparation Method
Native protein chromatographically purified from E. coli
Activity
>=30 units per mg protein (25°C, pH 8.0). One Unit hydrolyzes 1 umole of p-nitrophenol phosphate per minute at 25°C, pH 8.0.
Recommend Usage
Alkaline Phosphatase Assay
Storage Buffer
In 2.6 M ammonium sulfate, pH 8.0
Storage Instruction
Store at 4°C. This product is stable for at least 3 months.
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Applications
Enzyme Activity
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