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Last updated: 2020/11/29

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Ribonuclease A BioActive

  • Catalog # : P5303
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  • Specification
  • Product Description:
  • Solution. Purified. Aggregate-free (Monophoretic on native gel electrophoresis).
  • Host:
  • Bovine
  • Theoretical MW (kDa):
  • 13.7
  • Form:
  • Liquid
  • Preparation Method:
  • Native protein chromatographically purified from Bovine Pancreas
  • Activity:
  • >=3,000 units per mg protein. One Kunitz unit is the amount of enzyme required to cause an increase in absorbance of 1.0 at 260 nm at 37°C (pH 5.0) when yeast rRNA is hydrolyzed to acid-soluble oligonucleotides. 50 units are equivalent to 1 Kunitz unit.
  • Recommend Usage:
  • Ribonuclease Assay; RNA removal during DNA isolation; RNA sequence analysis; RNase protection assays; RNA quantification or mapping.
  • Storage Buffer:
  • In 100 mM phosphate buffer containing 0.1% v/v phenol as a preservative
  • Storage Instruction:
  • Store at -20°C.
  • Note:
  • The presence of 100 mM phosphate makes this preparation unsuitable for heat treatment.
  • Biological function:
  • Pancreatic ribonuclease (RNase) is an endoribonuclease. There are two forms, RNase A and RNase B, RNase B is the glycosylated form of RNase A.
  • Applications
  • Enzyme Activity
  • Application Image
  • Enzyme Activity
  • Gene Information
  • Gene Name:
  • RNASE1
  • Gene Alias:
  • RNS1
  • Gene Description:
  • ribonuclease
  • Other Designations:
  • ribonuclease A (pancreatic)
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