Lyophilized. Purified. A preparation equivalent to Cat. # P5301. Contains some aggregates as a result of lyophilization but shows no difference in specific activity.
Theoretical MW (kDa):
Native protein chromatographically purified from Bovine Pancreas
>=3,000 units per mg dry weight. One Kunitz unit is the amount of enzyme required to cause an increase in absorbance of 1.0 at 260 nm at 37°C (pH 5.0) when yeast rRNA is hydrolyzed to acid-soluble oligonucleotides. 50 units are equivalent to 1 Kunitz unit.
Ribonuclease Assay; RNA removal during DNA isolation; RNA sequence analysis; RNase protection assays; RNA quantification or mapping.
Store at 4°C on dry atmosphere.
Pancreatic ribonuclease (RNase) is an endoribonuclease. There are two forms, RNase A and RNase B, RNase B is the glycosylated form of RNase A.