Diaphorase (Cl. Kluyveri), NAD & NADP Removed
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Specification
Product Description
Lyophilized. Dialyzed. Pyridine nucleotides free.
Biological function
Diaphorase catalyzes the reaction of a reduced di- or tri-phosphopyridine nucleotide hydrogen donor with a hydrogen acceptor, usually a dye in the leucoform.
Host
Clostridium Species
Form
Lyophilized
Preparation Method
Native protein purified from Cl. kluyveri
Activity
>=25 units per mg dry weight. One Unit reduces 1 umole of DCPIP per minute at 25°C, pH 8.5.
Recommend Usage
Diaphorase Assay
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Avoid exposure to light after reconstitution.Note
Due to the presence of FMN (flavin mononucleotide), the enzyme, especially in solution, is light-sensitive.
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Applications
Enzyme Activity
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