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Diaphorase (Cl. Kluyveri), NAD & NADP RemovedBioActive

  • Catalog # : P5286
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  • Specification
  • Product Description:
  • Lyophilized. Dialyzed. Pyridine nucleotides free.
  • Host:
  • Clostridium Species
  • Form:
  • Lyophilized
  • Preparation Method:
  • Native protein purified from Cl. kluyveri
  • Activity:
  • >=25 units per mg dry weight. One Unit reduces 1 umole of DCPIP per minute at 25°C, pH 8.5.
  • Recommend Usage:
  • Diaphorase Assay
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Avoid exposure to light after reconstitution.
  • Note:
  • Due to the presence of FMN (flavin mononucleotide), the enzyme, especially in solution, is light-sensitive.
  • Biological function:
  • Diaphorase catalyzes the reaction of a reduced di- or tri-phosphopyridine nucleotide hydrogen donor with a hydrogen acceptor, usually a dye in the leucoform.
  • Applications
  • Enzyme Activity
  • Application Image
  • Enzyme Activity
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