Collagenase Type 4 (C. histolyticum)
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Specification
Product Description
Lyophilized. Dialyzed. 0.22um filtered. Contains lower tryptic activity levels to limit damage to membrane proteins and receptors but with normal to above normal collagenase activity.
Biological function
Collagenases are enzymes that break the peptide bonds in collagen.
Host
Clostridium Species
Form
Lyophilized
Preparation Method
Native protein purified from Clostridium histolyticum
Activity
>=160 units per mg dry weight. One Unit releases one micromole of L-leucine equivalents from collagen in 5 hours at 37°C, pH 7.5.
Recommend Usage
Collagenase Assay; Pancreatic islet primary isolation.
Storage Instruction
Store at 4°C on dry atmosphere.
For primary cell isolation, culture and tissue dissociation: Reconstituted in balanced salt solutions (such as Hank's, Earle's and others) at concentrations from 0.05% to 0.5% (w/v). For enzymetic assay: Reconstituted in 0.05 M TES buffer with 0.36 mM calcium chloride, pH 7.5 at a concentration of 1.0 mg/ml. -
Applications
Enzyme Activity
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