Collagenase Type 2 (C. histolyticum)
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Specification
Product Description
Lyophilized. Dialyzed. 0.22um filtered. Contains higher clostripain activity.
Biological function
Collagenases are enzymes that break the peptide bonds in collagen.
Host
Clostridium Species
Form
Lyophilized
Preparation Method
Native protein purified from Clostridium histolyticum
Activity
>=125 units per mg dry weight. One Unit releases one micromole of L-leucine equivalents from collagen in 5 hours at 37°C, pH 7.5.
Recommend Usage
Collagenase Assay; Bone, heart, liver, thyroid and salivary primary cell isolation.
Storage Instruction
Store at 4°C on dry atmosphere.
For primary cell isolation, culture and tissue dissociation: Reconstituted in balanced salt solutions (such as Hank's, Earle's and others) at concentrations from 0.05% to 0.5% (w/v). For enzymetic assay: Reconstituted in 0.05 M TES buffer with 0.36 mM calcium chloride, pH 7.5 at a concentration of 1.0 mg/ml. -
Applications
Enzyme Activity
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