Lyophilized. Dialyzed. The balance of collagenase, caseinase, clostripain and tryptic activities.
Native protein purified from Clostridium histolyticum
>=125 units per mg dry weight. One Unit releases one micromole of L-leucine equivalents from collagen in 5 hours at 37°C, pH 7.5.
Collagenase Assay; Epithelial, liver, lung and adrenal primary cell isolations.
Store at 4°C on dry atmosphere. For primary cell isolation, culture and tissue dissociation: Reconstituted in balanced salt solutions (such as Hank's, Earle's and others) at concentrations from 0.05% to 0.5% (w/v). For enzymetic assay: Reconstituted in 0.05 M TES buffer with 0.36 mM calcium chloride, pH 7.5 at a concentration of 1.0 mg/ml.