Collagenase Type 4 (C. histolyticum)
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
-
Specification
Product Description
Lyophilized. Dialyzed. Contains lower tryptic activity levels to limit damage to membrane proteins and receptors but with normal to above normal collagenase activity.
Biological function
Collagenases are enzymes that break the peptide bonds in collagen.
Host
Clostridium Species
Form
Lyophilized
Preparation Method
Native protein purified from Clostridium histolyticum
Activity
>=160 units per mg dry weight. One Unit releases one micromole of L-leucine equivalents from collagen in 5 hours at 37°C, pH 7.5.
Recommend Usage
Collagenase Assay; Pancreatic islet primary isolation.
Storage Instruction
Store at 4°C on dry atmosphere.
For primary cell isolation, culture and tissue dissociation: Reconstituted in balanced salt solutions (such as Hank's, Earle's and others) at concentrations from 0.05% to 0.5% (w/v). For enzymetic assay: Reconstituted in 0.05 M TES buffer with 0.36 mM calcium chloride, pH 7.5 at a concentration of 1.0 mg/ml. -
Applications
Enzyme Activity
-
Publication Reference
-
LC-MS-Based Targeted Metabolomics for FACS-Purified Rare Cells.
Katharina Schönberger, Michael Mitterer, Katharina Glaser, Manuel Stecher, Sebastian Hobitz, Dominik Schain-Zota, Konrad Schuldes, Tim Lämmermann, Angelika S Rambold, Nina Cabezas-Wallscheid, Joerg M Buescher.
Analytical Chemistry 2023 Mar; 95(9):4325.
Application:Enzyme, Mouse, Mouse skin.
-
One-to-one innervation of vocal muscles allows precise control of birdsong.
Iris Adam, Alyssa Maxwell, Helen Rößler, Emil B Hansen, Michiel Vellema, Jonathan Brewer, Coen P H Elemans.
Current Biology : CB 2021 Jul; 31(14):3115.
Application:Enzyme, Mouse, Mouse muscle.
-
LC-MS-Based Targeted Metabolomics for FACS-Purified Rare Cells.
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com