G6PDH (L. mesenteroides), NAD Assayed
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Specification
Product Description
Solution. Same as Cat.# P5265 except assayed using NAD. Phosphohexose isomerase, phosphogluconate dehydrogenase, adenylate kinase and creatine phosphokinase contaminant activities ≤ 0.011%, 0.002%, 0.0011% and 0.0011% respectively.
Biological function
Glucose-6-phosphate dehydrogenase is a cytosolic enzyme in the pentose phosphate pathway, which is a metabolic pathway that supplies reducing energy to cells by maintaining the level of the co-enzyme NADPH.
Host
Bacteria
Theoretical MW (kDa)
104
Form
Liquid
Preparation Method
Native protein chromatographically purified from Leuconostoc mesenteroides
Activity
>=360 NAD units per mg protein. One Unit reduces one micromole of NAD per minute at 37°C, pH 7.8, using glucose-6-phosphate as substrate.
Recommend Usage
Glucose-6-Phosphate Dehydrogenase Assay
Storage Buffer
In 3.7 M ammonium sulfate
Storage Instruction
Store at 4°C. This product is stable for at least 3 months.
Note
Care should be taken not to shake the solution as precipitation may result.
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Applications
Enzyme Activity
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