Solution. Phosphohexose isomerase, phosphogluconate dehydrogenase, adenylate kinase and creatine phosphokinase contaminant activities ≤ 0.02%, 0.003%, 0.002% and 0.002%, respectively.
Theoretical MW (kDa):
Native protein chromatographically purified from Leuconostoc mesenteroides
>=200 NADP units per mg protein. One Unit reduces one micromole of NADP per minute at 37°C, pH 7.8, using glucose-6-phosphate as substrate.
Glucose-6-Phosphate Dehydrogenase Assay
In 3.7 M ammonium sulfate
Store at 4°C. This product is stable for at least 3 months.
Care should be taken not to shake the solution as precipitation may result.
Glucose-6-phosphate dehydrogenase is a cytosolic enzyme in the pentose phosphate pathway, which is a metabolic pathway that supplies reducing energy to cells by maintaining the level of the co-enzyme NADPH.