Phosphodiesterase I (C. adamanteus)
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Specification
Product Description
Lyophilized.
Biological function
Venom exonuclease (Phosphodiesterase I) successively hydrolyzes 5'-mononucleotides from 3'-hydroxy-terminated ribo- and deoxyribo-oligonucleotides.
Host
Snake
Theoretical MW (kDa)
115
Form
Lyophilized
Preparation Method
Native protein purified from Crotalus adamanteus Venom according to the method of Williams, Sung and Laskowski, J. Biol. Chem., 236, 1130 (1961). Further treated to inactivate contaminating 5'-nucleotidase activity according to Sulkowski and Laskowski: Biochim. Biophys. Acta, 240, 443 (1961).
Activity
>=20 units per mg dry weight. One Unit hydrolyzes one micromole of p-nitrophenyl thymidine-5-phosphate per minute at 25°C, pH 8.9.
Recommend Usage
Phosphodiesterase I Assay; Structural and sequence studies of nucleic acids.
Storage Instruction
Store at -20°C.
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Applications
Enzyme Activity
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Publication Reference
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Effective delivery of STING agonist using exosomes suppresses tumor growth and enhances antitumor immunity.
Kathleen M. McAndrews, Sara P. Y. Che, Valerie S. LeBleu, Raghu Kalluri.
The Journal of Biological Chemistry 2021 Mar; 296:100523.
Application：Incubation, Human, 293T cells.
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Isotope-dilution mass spectrometry for exact quantification of noncanonical DNA nucleosides.
Traube FR, Schiffers S, Iwan K, Kellner S, Spada F, Müller M, Carell T.
Nature Protocols 2019 Jan; 14(1):283.
Application：Func, Human, Mouse, HEK 293T cells, Mouse cerebellum.
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Equilibrium and ultrafast kinetic studies manipulating electron transfer: a short-lived flavin semiquinone is not sufficient for electron bifurcation.
Hoben JP, Lubner CE, Ratzloff MW, Schut GJ, Nguyen DMN, Hempel KW, Adams MWW, King PW, Miller AF.
The Journal of Biological Chemistry 2017 Jun; 292(34):14039.
Application：Func, Pyrococcus furiosus, Recombinant protein.
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Effective delivery of STING agonist using exosomes suppresses tumor growth and enhances antitumor immunity.
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