Native protein purified from Crotalus adamanteus Venom according to the method of Williams, Sung and Laskowski, J. Biol. Chem., 236, 1130 (1961). Further treated to inactivate contaminating 5'-nucleotidase activity according to Sulkowski and Laskowski: Biochim. Biophys. Acta, 240, 443 (1961).
>=20 units per mg dry weight. One Unit hydrolyzes one micromole of p-nitrophenyl thymidine-5-phosphate per minute at 25°C, pH 8.9.
Phosphodiesterase I Assay; Structural and sequence studies of nucleic acids.
Store at -20°C.
Venom exonuclease (Phosphodiesterase I) successively hydrolyzes 5'-mononucleotides from 3'-hydroxy-terminated ribo- and deoxyribo-oligonucleotides.