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Last updated: 2020/6/28

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Papain (Papaya), 2x BioActive

  • Catalog # : P5246
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  • Specification
  • Product Description:
  • Solution. 2x crystalline. It is recommended that the enzyme be 0.22um filtered after reconstitution and prior to use.
  • Host:
  • Plants
  • Theoretical MW (kDa):
  • 23.4
  • Form:
  • Liquid
  • Preparation Method:
  • Native protein purified from Papaya Latex
  • Activity:
  • Activates to at least 20 units per mg protein. One Unit hydrolyzes one micromole of benzoyl-L-arginine ethyl ester per minute at 25°C, pH 6.2, after activation in a solution containing 1.1 mM EDTA, 0.067 mM mercaptoethanol and 5.5 mM cysteine-HCl for 30 minutes.
  • Recommend Usage:
  • Papain Assay; Cell isolation (more gentle than other proteases); Protein structural studies; Peptide mapping; Red cell surface modification for antibody screening or identification; Fab preparation from IgG and IgM antibodies; Solubilization of integral membrane proteins; Production of glycopeptides from purified proteoglycans; Enzymatic wound debridement.
  • Storage Buffer:
  • In 50 mM sodium acetate, pH 4.5
  • Storage Instruction:
  • Store at 4°C. This product is stable for at least 3 months.
  • Note:
  • It should be incubated in the activation solution (1.1 mM EDTA, 0.067 mM mercaptoethanol and 5.5 mM cysteine-HCl) for 30 minutes before use to ensure full activity.
  • Biological function:
  • Papain is a cysteine hydrolase that is stable and active under a wide range of conditions.
  • Applications
  • Enzyme Activity
  • Application Image
  • Enzyme Activity
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