Solution. 2x crystalline. It is recommended that the enzyme be 0.22um filtered after reconstitution and prior to use.
Theoretical MW (kDa):
Native protein purified from Papaya Latex
Activates to at least 20 units per mg protein. One Unit hydrolyzes one micromole of benzoyl-L-arginine ethyl ester per minute at 25°C, pH 6.2, after activation in a solution containing 1.1 mM EDTA, 0.067 mM mercaptoethanol and 5.5 mM cysteine-HCl for 30 minutes.
Papain Assay; Cell isolation (more gentle than other proteases); Protein structural studies; Peptide mapping; Red cell surface modification for antibody screening or identification; Fab preparation from IgG and IgM antibodies; Solubilization of integral membrane proteins; Production of glycopeptides from purified proteoglycans; Enzymatic wound debridement.
In 50 mM sodium acetate, pH 4.5
Store at 4°C. This product is stable for at least 3 months.
It should be incubated in the activation solution (1.1 mM EDTA, 0.067 mM mercaptoethanol and 5.5 mM cysteine-HCl) for 30 minutes before use to ensure full activity.
Papain is a cysteine hydrolase that is stable and active under a wide range of conditions.