Papain (Papaya), 2x
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Specification
Product Description
Lyophilized. 2x crystalline. It is recommended that the enzyme be 0.22um filtered after reconstitution and prior to use.
Biological function
Papain is a cysteine hydrolase that is stable and active under a wide range of conditions.
Host
Plants
Theoretical MW (kDa)
23.4
Form
Lyophilized
Preparation Method
Native protein purified from Papaya Latex
Activity
Activates to at least 15 units per mg protein. One Unit hydrolyzes one micromole of benzoyl-L-arginine ethyl ester per minute at 25°C, pH 6.2, after activation in a solution containing 1.1 mM EDTA, 0.067 mM mercaptoethanol and 5.5 mM cysteine-HCl for 30 minutes.
Recommend Usage
Papain Assay; Cell isolation (more gentle than other proteases); Protein structural studies; Peptide mapping; Red cell surface modification for antibody screening or identification; Fab preparation from IgG and IgM antibodies; Solubilization of integral membrane proteins; Production of glycopeptides from purified proteoglycans; Enzymatic wound debridement.
Storage Instruction
Store at 4°C.
Note
It should be incubated in the activation solution (1.1 mM EDTA, 0.067 mM mercaptoethanol and 5.5 mM cysteine-HCl) for 30 minutes before use to ensure full activity.
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Applications
Enzyme Activity
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