Solution. 2x crystalline. The enzyme must be diluted and 0.22 um filtered before use.
Theoretical MW (kDa):
Native protein purified from Porcine Pancreas
>=3 units per mg protein. One Unit cleaves one micromole of N-succinyl-L-alanyl-L-alanyl-L-alanine-p-nitroanilide per minute at 25°C, pH 8.0.
Elastase Assay; Isolation of Type II lung cells.
Store at 4°C. Do not freeze. This product is stable for at least 3 months.
Elastase is a serine protease similar to trypsin and chymotrypsin that also hydrolyses amides and ester, it is unique among proteases in its ability to hydrolyze native elastin, a substrate not attacked by trypsin, chymotrypsin or pepsin.