Clostripain (Cl. Histolyticum)
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Specification
Product Description
Lyophilized. Dialyzed. Pre-activated.
Biological function
Clostripain is unique in its specificity for the carboxyl peptide bond of arginine and its dependence on thiol and calcium ions.
Host
Clostridium Species
Form
Lyophilized
Preparation Method
Native protein chromatographically purified from Clostridium histolyticum
Activity
>=50 units per mg dry weight. One Unit hydrolyzes one micromole of N-benzoyl-L-arginine ethyl ester per minute at 25°C, pH 7.6, in the presence of dithiothreitol.
Recommend Usage
Clostripain Assay; Peptide mapping; Sequence analysis; Cell isolation; Hydrolysis/condensation of amide bonds; Peptide synthesis.
Storage Instruction
Store at 4°C.
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Applications
Enzyme Activity
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Publication Reference
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A hotspot for posttranslational modifications on the androgen receptor dimer interface drives pathology and anti-androgen resistance.
Andrea Alegre-Martí, Alba Jiménez-Panizo, Adrián Martínez-Tébar, Coralie Poulard, M Núria Peralta-Moreno, Montserrat Abella, Rosa Antón, Marcos Chiñas, Ulrich Eckhard, Josep M Piulats, Ana M Rojas, Juan Fernández-Recio, Jaime Rubio-Martínez, Muriel Le Romancer, Álvaro Aytes, Pablo Fuentes-Prior, Eva Estébanez-Perpiñá.
Science Advances 2023 Mar; 9(11):eade2175.
Application：Enzyme, Recombinant proteins.
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A hotspot for posttranslational modifications on the androgen receptor dimer interface drives pathology and anti-androgen resistance.
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