Acid phosphatase (Wheat Germ)
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Specification
Product Description
Lyophilized. Partially purified. Also active as a lipase.
Biological function
Acid phosphatase is an esterase with broad activity at an optimal pH below 7.0. There are three isozymes, EI, EII, and EIII of similar molecular weight (55 kDa± 5 kDa). Their optimum pH's are 5.5, 4.5, and 4.0 respectively.
Host
Plants
Theoretical MW (kDa)
50~60
Form
Lyophilized
Preparation Method
Native protein partially purified from Wheat Germ to the 0.35-0.55 fraction by the method described by Singer, J. Biol. Chem., 174, 11 (1948)
Activity
>=0.15 units per mg dry weight. One Unit hydrolyzes one micromole of o-carboxyphenyl phosphate per minute at 25°C, pH 5.0.
Recommend Usage
Acid Phosphatase Assay
Storage Instruction
Store at -20°C.
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Applications
Enzyme Activity
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