DNase
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Specification
Product Description
Lyophilized. 0.22 um filtered.
Biological function
Bovine pancreatic deoxyribonuclease is an endonuclease which splits phosphodiester linkages, preferentially adjacent to a pyrimidine nucleotide yielding polynucleotides with free hydroxyl group at the 3' position and phosphate group at the 5' position.
Host
Bovine
Theoretical MW (kDa)
31.3
Form
Lyophilized
Preparation Method
Native protein purified from Bovine Pancreas
Activity
>=1,000 units per vial. One Unit causes an increase in absorbance at 260 nm of 0.001 per minute per ml at 25°C when acting upon highly polymerized DNA at pH 5.0.
Recommend Usage
Deoxyribonuclease I Assay; DNA removal in primary cell isolation to decreases viscosity providing better yields; DNA removal in bioprocessing applications.
Storage Instruction
Store at 4°C.
Reconstituted with 0.5 ml of Earle's Balanced Salt Solution (EBSS) or equivalent yields a solution of 2000 units/ml (1 mg/ml). -
Applications
Enzyme Activity
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Gene Info — DNASE1
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