Ifng (Rat) Recombinant Protein
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Specification
Product Description
Rat Ifng (P01581) recombinant protein expressed in Escherichia coli.
Sequence
QGTLIESLESLKNYFNSSSMDAMEGKSLLLDIWRNWQKDGNTKILESQIISFYLRLFEVLKDNQAISNNISVIESHLITNFFSNSKAKKDAFMSIAKFEVNNPQIQHKAVNELIRVIHQLSPESSLRKRKRSRC
Host
Escherichia coli
Theoretical MW (kDa)
16
Form
Lyophilized
Preparation Method
Escherichia coli expression system
Purification
Ion exchange column and HPLC reverse phase column
Purity
> 90% by SDS-PAGE and HPLC
Endotoxin Level
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
Activity
The ED(50) was determined by the cytopathic inhibition assay with murine L929 cells infected by EMC virus, and was found to be < 1 ng/mL, corresponding to a specific activity of > 1 x 106 units/mg.
Storage Buffer
Lyophilized from PBS, pH 7.0
Storage Instruction
Store at -20°C on dry atmosphere.
After reconstitution with sterilized water, store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Functional Study
SDS-PAGE
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Gene Info — Ifng
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