trxA (Escherichia coli) Recombinant protein
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Specification
Product Description
Escherichia coli trxA (ACB04810, 2 a.a. - 109 a.a.) partial recombinant protein with His tag expressed in Escherichia coli.
Sequence
MHHHHHHMGSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILDEIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKEFLDANLAGS
Host
Escherichia coli
Theoretical MW (kDa)
34.6
Form
Liquid
Preparation Method
Escherichia coli expression system
Purification
Conventional Chromatography
Concentration
0.5 mg/mL
Purity
> 85% by SDS-PAGE
Activity
Specific activity is 16~18 A650/min/mg and was obtained by measuring the increase of insulin precipitation in absorbance at 650 nm resulting from the reduction of insulin.
Quality Control Testing
15% SDS-PAGE Stained with Coomassie Blue
Storage Buffer
In 20 mM Tris-HCl buffer, pH 8.0 (1 mM DTT, 10% glycerol).
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Functional Study
SDS-PAGE
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Gene Info — trxA
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