Specific activity is 8-10 units/mL obtained by measuring the increase of NADPH in absorbance at 340 nm resulting from the reduction of NAD or NADP. One unit oxidizes 1.0 umole D-glucose-6-phosphate to 6-phospho-D-gluconate per min in the presence of beta-
Quality Control Testing:
15% SDS-PAGE Stained with Coomassie Blue
In 50 mM MES, pH 6.0 (0.1 mM PMSF, 2 mM EDTA, 0.5 mM DTT, 10% glycerol)
Store at -20°C. For long term storage store at -80°C. Aliquot to avoid repeated freezing and thawing.