slyD (Escherichia coli) Recombinant protein
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Specification
Product Description
Escherichia coli slyD (NP_755987, 1 a.a. - 196 a.a.) full-length recombinant protein expressed in Escherichia coli.
Sequence
MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISGLETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQGPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHHDHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH
Host
Escherichia coli
Theoretical MW (kDa)
54
Form
Liquid
Preparation Method
Escherichia coli expression system
Purification
Conventional Chromatography
Concentration
1 mg/mL
Purity
> 95% by SDS-PAGE
Activity
Specific activity is > 220 nmoles/min/mg, and is defined as the amount of enzyme that cleaves 1umole of suc-AAFP-pNA per minute at 25°C in Tris-HCl pH 8.0 using chymotrypsin.
Quality Control Testing
15% SDS-PAGE Stained with Coomassie Blue
Storage Buffer
In 20 mM Tris, pH 7.5.
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Functional Study
SDS-PAGE
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Gene Info — slyD
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