The activity is determined by the ability to induce proliferation of TF-1 cells and is 10-15 ng/mL.
Quality Control Testing:
1 ug/lane in 4-20% Tris-Glycine gel stained with Coomassie Blue
Lane 1: non-reducing conditions Lane 2: reducing conditions
Storage Buffer:
No additive
Storage Instruction:
Store at -20°C on dry atmosphere. After reconstitution with sterilized 10 mM acetic acid, store at -20°C. Aliquot to avoid repeated freezing and thawing.
Note:
Result of activity analysis
Serial dilutions of rat Kitlg, starting at 250 ng/mL, were added to TF-1 cells growing in GM-SCF free media. Cell proliferation was measure after 63 hours and the linear portion of the curve was us used to calculate the ED50.