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Last updated: 2017/7/23

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Lep (Rat) Recombinant ProteinBioActive

  • Catalog # : P4847
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  • Specification
  • Product Description:
  • Rat Lep recombinant protein expressed in Escherichia coli.
  • Sequence:
  • MVPIHKVQDDTKTLIKTIVTRINDISHTQSVSARQRVTGLDFIPGLHPILSLSKMDQTLAVYQQILTSLPSQNVLQIAHDLENLRDLLHLLAFSKSCSLPQTRGLQKPESLDGVLEASLYSTEVVALSRLQGSLQDILQQLDLSPEC
  • Host:
  • Escherichia coli
  • Theoretical MW (kDa):
  • 16.2
  • Form:
  • Lyophilized
  • Preparation Method:
  • Escherichia coli expression system
  • Endotoxin Level:
  • < 0.1 EU/ug
  • Activity:
  • The activity is determined by the leptin-dependent stimulation of mouse BaF3 indicator cells. The expected ED50 for this effect is 4-6.7 ng/mL.
  • Quality Control Testing:
  • 1 ug/lane in 4-20% Tris-Glycine gel stained with Coomassie Blue

    QC Testing of P4847
    Lane 1: non-reducing conditions
    Lane 2: reducing conditions
  • Storage Buffer:
  • Lyophilized from 0.1% TFA
  • Storage Instruction:
  • Store at -20°C on dry atmosphere.
    After reconstitution with sterilized water, store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • Result of activity analysis

  • Serial dilutions of rat Lep were added to Baf/3 cells. After 44 hours cell proliferation was measured and the linear portion of the curve was used to determine the ED50.


  • Applications
  • Functional Study
  • SDS-PAGE
  • Application Image
  • Functional Study
  • SDS-PAGE
  • Gene Information
  • Gene Name:
  • Lep
  • Gene Alias:
  • OB,obese
  • Gene Description:
  • leptin
  • Other Designations:
  • Obesity (murine homolog, leptin)
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