The activity is determined by the leptin-dependent stimulation of mouse BaF3 indicator cells. The expected ED50 for this effect is 4-6.7 ng/mL.
Quality Control Testing:
1 ug/lane in 4-20% Tris-Glycine gel stained with Coomassie Blue
Lane 1: non-reducing conditions Lane 2: reducing conditions
Storage Buffer:
Lyophilized from 0.1% TFA
Storage Instruction:
Store at -20°C on dry atmosphere. After reconstitution with sterilized water, store at -20°C. Aliquot to avoid repeated freezing and thawing.
Note:
Result of activity analysis
Serial dilutions of rat Lep were added to Baf/3 cells. After 44 hours cell proliferation was measured and the linear portion of the curve was used to determine the ED50.