Il17a/Il17f (Rat) Recombinant Protein
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Specification
Product Description
Rat Il17a/Il17f (heterodimer) recombinant protein expressed in Escherichia coli.
Sequence
Il17a: MAVLIPQSSVCPNAEANNFLQNVKVNLKVLNSLSSKASSRRPSDYLNRSTSPWTLSRNEDPDRYPSVIWEAQCRHQRCVNAEGKLDHHMNSVLIQQEILVLKREPEKCPFTFRVEKMLVGVGCTCVSSIVRHAS
Il17f: MARRNPKVGLSALQKAGNCPPLEDNSVRVDIRIFNQNQGISVPRDFQNRSSSPWDYNITRDPDRFPSEIAEAQCRHSGCINAQGQEDGSMNSVPIQQEILVLRREPQGCSNSFRLEKMLIKVGCTCVTPIVHHAAHost
Escherichia coli
Theoretical MW (kDa)
30.7
Form
Lyophilized
Preparation Method
Escherichia coli expression system
Endotoxin Level
< 0.1 EU/ug
Activity
The activity is determined by the ability to induce IL-6 production from NIH-3T3 cells. The expected ED50 for this effect is 15.3-23 ng/mL.
Quality Control Testing
1 ug/lane in 4-20% Tris-Glycine gel stained with Coomassie Blue
Lane 1: non-reducing conditions
Lane 2: reducing conditionsStorage Buffer
No additive
Storage Instruction
Store at -20°C on dry atmosphere.
After reconstitution with sterilized water, store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Functional Study
SDS-PAGE
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Gene Info — Il17a
Entrez GeneID
301289Protein Accession#
Q61453 (Gene ID : 301289);Q5BJ95 (Gene ID : 301291)Gene Name
Il17a
Gene Alias
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Gene Description
interleukin 17A
Gene Ontology
HyperlinkOther Designations
Il17|IL-17|CTLA-8|IL-17A
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Gene Info — Il17f
Entrez GeneID
301291Protein Accession#
Q61453 (Gene ID : 301289);Q5BJ95 (Gene ID : 301291)Gene Name
Il17f
Gene Alias
MGC114402
Gene Description
interleukin 17F
Gene Ontology
HyperlinkOther Designations
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