The activity is determined by the dose-dependent induction of IL-6 production in cultured mouse NIH 3T3 fibroblasts. The expected ED50 for this effect is 0.44-0.66 ng/mL.
Quality Control Testing:
1 ug/lane in 4-20% Tris-Glycine gel stained with Coomassie Blue
Lane 1: non-reducing conditions Lane 2: reducing conditions
Storage Buffer:
Lyophilized from 10 mM sodium citrate, pH 3.0
Storage Instruction:
Store at -20°C on dry atmosphere. After reconstitution with sterilized water, store at -20°C. Aliquot to avoid repeated freezing and thawing.
Note:
Result of activity analysis
Serial dilutions of rat Il17a (starting at 2.5 ug/mL) were added to NIH 3T3 cells. After 48 hours, production of mouse IL-6 was measured and the linear portion of the curve was us used to calculate the ED50.