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BRAF (V600E) (Human) Recombinant ProteinBioActive

  • Catalog # : P4833
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  • Specification
  • Product Description:
  • Human BRAF (NM_004333.2, 417 a.a. - 766 a.a.) V600E mutant partial protein with GST-His tag expressed in Sf9 cells.
  • Host:
  • Insect
  • Theoretical MW (kDa):
  • 69.157
  • Form:
  • Liquid
  • Preparation Method:
  • Insect cell (Sf9) expression system
  • Purification:
  • GST affinity chromatography
  • Concentration:
  • 0.190 ug/uL
  • Activity:
  • 197 pmol/ug x min
  • Quality Control Testing:
  • 2 ug/lane SDS-PAGE Stained with Coomassie Blue

    QC Testing of P4833
  • Storage Buffer:
  • In 50 mM Hepes, 100 mM NaCl, pH 7.5 (5 mM DTT, 4 mM reduced glutathione, 20% glycerol)
  • Storage Instruction:
  • Store at -80°C.
    Aliquot to avoid repeated freezing and thawing
  • Note:
  • Result of activity analysis

  • Applications
  • Functional Study
  • SDS-PAGE
  • Application Image
  • Functional Study
  • SDS-PAGE
  • Gene Information
  • Entrez GeneID:
  • 673
  • Gene Name:
  • BRAF
  • Gene Alias:
  • B-RAF1,BRAF1,FLJ95109,MGC126806,MGC138284,RAFB1
  • Gene Description:
  • v-raf murine sarcoma viral oncogene homolog B1
  • Gene Summary:
  • This gene encodes a protein belonging to the raf/mil family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. Mutations in this gene have also been associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung. A pseudogene, which is located on chromosome X, has been identified for this gene. [provided by RefSeq
  • Other Designations:
  • 94 kDa B-raf protein,B-Raf proto-oncogene serine/threonine-protein kinase (p94),Murine sarcoma viral (v-raf) oncogene homolog B1
  • Related Disease
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