Pdgfa/Pdgfa (Mouse) Recombinant Protein
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Result of activity analysis
Result of activity analysis
Serial dilutions of mouse PDGF-AA, starting at 100 ng/mL, were added to NIH 3T3 cells. After 40 hours, cell viability was measured and the linear portion of the curve was us used to calculate the ED50 .
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Specification
Product Description
Mouse Pdgfa (homodimer) recombinant protein expressed in Escherichia coli.
Sequence
MSIEEAVPAVCKTRTVIYEIPRSQVDPTSANFLIWPPCVEVKRCTGCCNTSSVKCQPSRVHHRSVKVAKVEYVRKKPKLKEVQVRLEEHLECACATSNLNPDHREEETGRRRESGKNRKRKRLKPT
Host
Escherichia coli
Theoretical MW (kDa)
28.9
Form
Lyophilized
Preparation Method
Escherichia coli expression system
Endotoxin Level
< 0.1 EU/ug
Activity
The activity is determined by the dose-dependent proliferation of Balb/c 3T3 cells. The expected ED50 for this effect is 3.4-5 ng/mL.
Storage Buffer
No additive
Storage Instruction
Store at -20°C on dry atmosphere.
After reconstitution with sterilized water, store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of activity analysis
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Applications
Functional Study
SDS-PAGE
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Gene Info — Pdgfa
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