The activity is determined by the dose-dependent proliferation of Balb/c 3T3 cells. The expected ED50 for this effect is 3.4-5 ng/mL.
Storage Buffer:
No additive
Storage Instruction:
Store at -20°C on dry atmosphere. After reconstitution with sterilized water, store at -20°C. Aliquot to avoid repeated freezing and thawing.
Note:
Result of activity analysis
Serial dilutions of mouse PDGF-AA, starting at 100 ng/mL, were added to NIH 3T3 cells. After 40 hours, cell viability was measured and the linear portion of the curve was us used to calculate the ED50 .