Fgf9 (Rat) Recombinant Protein
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Lane 1: non-reducing conditions
Lane 2: reducing conditions
Result of activity analysis
Result of activity analysis
3T3 cells were cultured with 0 to 100 ng/mL rat Fgf9. Cell proliferation was measured after 42 hours and the linear portion of the curve was us used to calculate the ED50.
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Specification
Product Description
Rat Fgf9 recombinant protein expressed in Escherichia coli.
Sequence
MPLGEVGSYFGVQDAVPFGNVPVLPVDSPVLLSDHLGQSEAGGLPRGPAVTDLDHLKGILRRRQLYCRTGFHLEIFPNGTIQGTRKDHSRFGILEFISIAVGLVSIRGVDSGLYLGMNEKGELYGSEKLTQECVFREQFEENWYNTYSSNLYKHVDTGRRYYVALNKDGTPREGTRTKRHQKFTHFLPRPVDPDKVPELYKDILSQS
Host
Escherichia coli
Theoretical MW (kDa)
23.3
Form
Lyophilized
Preparation Method
Escherichia coli expression system
Endotoxin Level
< 0.1 EU/ug
Activity
The activity is determined by the dose-dependent proliferation of 3T3 cells. The expected ED50 for this effect is 0.9-1.4 ng/mL.
Quality Control Testing
1 ug/lane in 4-20% Tris-Glycine gel stained with Coomassie Blue
Lane 1: non-reducing conditions
Lane 2: reducing conditionsStorage Buffer
Lyophilized from 10 mM NaP, 75 mM (NH 4 )2 SO4, pH 7.5
Storage Instruction
Store at -20°C on dry atmosphere.
After reconstitution with sterilized water, store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of activity analysis
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Applications
Functional Study
SDS-PAGE
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Gene Info — Fgf9
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