WEE1 (Human) Recombinant Protein
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Result of activity analysis
Result of activity analysis
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Specification
Product Description
Human WEE1 (X62048, 250 a.a. - 646 a.a.) partial Recombinant protein with GST-His tag expressed in Sf9 cells.
Host
insect
Theoretical MW (kDa)
74.902
Form
Liquid
Preparation Method
Insect cell (Sf9) expression system
Purification
One-step affinity purification using GSH-agarose
Concentration
0.142 ug/uL
Activity
2 pmol/ug x min
Quality Control Testing
2 ug/lane SDS-PAGE Stained with Coomassie Blue
Storage Buffer
In 50 mM Tris-HCl, 100 mM NaCl, pH 8.0 (5 mM DTT, 4 mM reduced glutathione, 20% glycerol)
Storage Instruction
Store at -80°C.
Aliquot to avoid repeated freezing and thawingNote
Result of activity analysis
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Applications
Functional Study
SDS-PAGE
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Gene Info — WEE1
Entrez GeneID
7465Protein Accession#
X62048Gene Name
WEE1
Gene Alias
DKFZp686I18166, FLJ16446, WEE1A, WEE1hu
Gene Description
WEE1 homolog (S. pombe)
Omim ID
193525Gene Ontology
HyperlinkGene Summary
This gene encodes a nuclear protein, which is a tyrosine kinase belonging to the Ser/Thr family of protein kinases. This protein catalyzes the inhibitory tyrosine phosphorylation of CDC2/cyclin B kinase, and appears to coordinate the transition between DNA replication and mitosis by protecting the nucleus from cytoplasmically activated CDC2 kinase. [provided by RefSeq
Other Designations
WEE1+ homolog|wee1 tyrosine kinase|wee1-like protein kinase
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Interactome
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Pathway
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Disease
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