STK16 (Human) Recombinant Protein

Catalog # P4788
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Size:100 ug
Price: USD $ 1,932.00
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QC Test

Result of activity analysis
Note

Result of activity analysis

Result of activity analysis

Determination of Vmax and KM value for ATP
Assay conditions:
60 mM HEPES-NaOH, pH 7.5
3 mM MgCl2
3 mM MnCl2
3 uM Na-orthovanadate
1.2 mM DTT
50 ug/mL PEG20.000
ATP (variable)
Substrate: Casein 200 ug/mL
Kinase: 2 ug/mL

Filter binding assay:
MSFC membrane (Millipore)

  • Specification

    Product Description

    Human STK16 (NP_001008910.1, 1 a.a. - 305 a.a.) full-length recombinant protein with GST-His tag with a Thrombin cleavage site at N-terminal expressed in Sf9 insect cells.Bioactive Protein,Bioactive Proteins,Bioactive,Active,Functional Protein,Functional Proteins,Full-Length Protein,Full-Length Proteins,Full-Length,Full Length,FullLength

    Sequence

    MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDPMGHHHHHHGRRRASVAAGILVPRGSPGLDGIYARGIQMGHALCVCSRGTVIIDNKRYLFIQKLGEGGFSYVDLVEGLHDGHFYALKRILCHEQQDREEAQREADMHRLFNHPNILRLVAYCLRERGAKHEAWLLLPFFKRGTLWNEIERLKDKGNFLTEDQILWLLLGICRGLEAIHAKGYAHRDLKPTNILLGDEGQPVLMDLGSMNQACIHVEGSRQALTLQDWAAQRCTISYRAPELFSVQSHCVIDERTDVWSLGCVLYAMMFGEGPYDMVFQKGDSVALAVQNQLSIPQSPRHSSALRQLLNSMMTVDPHQRPHIPLLLSQLEALQPPAPGQHTTQI

    Host

    insect

    Theoretical MW (kDa)

    64.393

    Form

    Liquid

    Preparation Method

    Insect cell (Sf9) expression system

    Purification

    GST-Affinity Chromatography

    Concentration

    1.020 ug/uL

    Activation

    This kinase was not activated by special procedures.

    Activity

    Specific kinase activity (Pi transfer): 3.5 pmol/ug x min
    ATP-KM: 0.3 uM

    Quality Control Testing

    2 ug/lane SDS-PAGE Stained with Coomassie Blue

    Storage Buffer

    In 50 mM Tris-HCl, 100 mM NaCl, pH 8.0 (5 mM DTT, 15 mM reduced glutathione, 20% glycerol).

    Storage Instruction

    Store at -80°C.
    Aliquot to avoid repeated freezing and thawing

    Note

    Result of activity analysis

  • Applications

    Functional Study

    SDS-PAGE

  • Gene Info — STK16

    Entrez GeneID

    8576

    Protein Accession#

    NP_001008910.1

    Gene Name

    STK16

    Gene Alias

    FLJ39635, KRCT, MPSK, PKL12, TSF1

    Gene Description

    serine/threonine kinase 16

    Omim ID

    604719

    Gene Ontology

    Hyperlink

    Other Designations

    protein kinase expressed in day 12 fetal liver

  • Interactome
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Contact Info
  • +1-909-264-1399
    +1-909-992-0619
    Toll Free : +1-877-853-6098
  • +1-909-992-3401
Product Inquiry
Bulk Inquiry
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