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STK16 (Human) Recombinant Protein BioActive

  • Catalog # : P4788
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  • Specification
  • Product Description:
  • Human STK16 (NP_001008910.1, 1 a.a. - 305 a.a.) full-length recombinant protein with GST-His tag with a Thrombin cleavage site at N-terminal expressed in Sf9 insect cells.
  • Sequence:
  • MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDPMGHHHHHHGRRRASVAAGILVPRGSPGLDGIYARGIQMGHALCVCSRGTVIIDNKRYLFIQKLGEGGFSYVDLVEGLHDGHFYALKRILCHEQQDREEAQREADMHRLFNHPNILRLVAYCLRERGAKHEAWLLLPFFKRGTLWNEIERLKDKGNFLTEDQILWLLLGICRGLEAIHAKGYAHRDLKPTNILLGDEGQPVLMDLGSMNQACIHVEGSRQALTLQDWAAQRCTISYRAPELFSVQSHCVIDERTDVWSLGCVLYAMMFGEGPYDMVFQKGDSVALAVQNQLSIPQSPRHSSALRQLLNSMMTVDPHQRPHIPLLLSQLEALQPPAPGQHTTQI
  • Host:
  • Insect
  • Theoretical MW (kDa):
  • 64.393
  • Form:
  • Liquid
  • Preparation Method:
  • Insect cell (Sf9) expression system
  • Purification:
  • GST-Affinity Chromatography
  • Concentration:
  • 1.020 ug/uL
  • Activity:
  • Specific kinase activity (Pi transfer): 3.5 pmol/ug x min
    ATP-KM: 0.3 uM
  • Quality Control Testing:
  • 2 ug/lane SDS-PAGE Stained with Coomassie Blue

    QC Testing of P4788
  • Storage Buffer:
  • In 50 mM Tris-HCl, 100 mM NaCl, pH 8.0 (5 mM DTT, 15 mM reduced glutathione, 20% glycerol).
  • Storage Instruction:
  • Store at -80°C.
    Aliquot to avoid repeated freezing and thawing
  • Note:
  • Result of activity analysis
  • Determination of Vmax and KM value for ATP
    Assay conditions:
    60 mM HEPES-NaOH, pH 7.5
    3 mM MgCl2
    3 mM MnCl2
    3 uM Na-orthovanadate
    1.2 mM DTT
    50 ug/mL PEG20.000
    ATP (variable)
    Substrate: Casein 200 ug/mL
    Kinase: 2 ug/mL

    Filter binding assay:
    MSFC membrane (Millipore)


  • Activation:
  • This kinase was not activated by special procedures.
  • Applications
  • Functional Study
  • SDS-PAGE
  • Application Image
  • Functional Study
  • SDS-PAGE
  • Gene Information
  • Entrez GeneID:
  • 8576
  • Gene Name:
  • STK16
  • Gene Alias:
  • FLJ39635,KRCT,MPSK,PKL12,TSF1
  • Gene Description:
  • serine/threonine kinase 16
  • Other Designations:
  • protein kinase expressed in day 12 fetal liver
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