PLK4 (Human) Recombinant Protein
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Result of activity analysis
Result of activity analysis
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Specification
Product Description
Human PLK4 (NM_014264.2, 1 a.a. - 970 a.a.) full-length recombinant protein with GST-His tag expressed in Sf9 cells.
Host
insect
Theoretical MW (kDa)
138.568
Form
Liquid
Preparation Method
Insect cell (Sf9) expression system
Purification
One-step affinity purification using GSH agarose
Concentration
0.050 ug/uL
Activity
6 pmol/ug x min
Quality Control Testing
2 ug/lane SDS-PAGE Stained with Coomassie Blue
Storage Buffer
In 50 mM Hepes, 100 mM NaCl, pH 7.5. (5 mM DTT, 4 mM reduced glutathione, 20% glycerol)
Storage Instruction
Store at -80°C.
Aliquot to avoid repeated freezing and thawingNote
Result of activity analysis
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Applications
Functional Study
SDS-PAGE
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Gene Info — PLK4
Entrez GeneID
10733Protein Accession#
NM_014264.2Gene Name
PLK4
Gene Alias
SAK, STK18
Gene Description
polo-like kinase 4 (Drosophila)
Omim ID
605031Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the polo family of serine/threonine protein kinases. The protein localizes to centrioles, complex microtubule-based structures found in centrosomes, and regulates centriole duplication during the cell cycle. [provided by RefSeq
Other Designations
Snk akin kinase|polo-like kinase 4|serine/threonine kinase 18|serine/threonine protein kinase SAK
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Interactome
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Disease
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Publication Reference
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Role for polo-like kinase 4 in mediation of cytokinesis.
Press MF, Xie B, Davenport S, Zhou Y, Guzman R, Nolan GP, O'Brien N, Palazzolo M, Mak TW, Brugge JS, Slamon DJ.
PNAS 2019 Jun; 116(23):11309.
Application：Func, Antibody.
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Role for polo-like kinase 4 in mediation of cytokinesis.
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