PRKCQ (Human) Recombinant Protein
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Specification
Product Description
Human PRKCQ (NM_006257, 1 a.a. - 706 a.a.) full-length recombinant protein with GST tag expressed in Sf9 cells.
Host
insect
Theoretical MW (kDa)
109.677
Form
Liquid
Preparation Method
Insect cell (Sf9) expression system
Purification
One-step affinity purification using GSH agarose
Concentration
0.273 ug/uL
Activity
166 pmol/ug x min
Quality Control Testing
2 ug/lane SDS-PAGE Stained with Coomassie Blue
Storage Buffer
In 50 mM Tris-HCl, 100 mM NaCl, pH 8.0. (5 mM DTT, 4 mM reduced glutathione, 20% glycerol)
Storage Instruction
Store at -80°C.
Aliquot to avoid repeated freezing and thawingNote
Result of activity analysis
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Applications
Functional Study
SDS-PAGE
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Gene Info — PRKCQ
Entrez GeneID
5588Protein Accession#
NM_006257Gene Name
PRKCQ
Gene Alias
MGC126514, MGC141919, PRKCT, nPKC-theta
Gene Description
protein kinase C, theta
Omim ID
600448Gene Ontology
HyperlinkGene Summary
Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role. The protein encoded by this gene is one of the PKC family members. It is a calcium-independent and phospholipid-dependent protein kinase. This kinase is important for T-cell activation. It is required for the activation of the transcription factors NF-kappaB and AP-1, and may link the T cell receptor (TCR) signaling complex to the activation of the transcription factors. [provided by RefSeq
Other Designations
OTTHUMP00000019053|OTTHUMP00000043364|OTTHUMP00000043365
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