PRKD3 (Human) Recombinant Protein
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Specification
Product Description
Human PRKD3 (AB015982.2, 3 a.a. - 890 a.a.) partial recombinant protein expressed in Sf9 cells.
Host
insect
Theoretical MW (kDa)
100.793
Form
Liquid
Preparation Method
Insect cell (Sf9) expression system
Purification
GST affinity chromatography
Concentration
0.122 ug/uL
Activity
16 pmol/ug x min
Quality Control Testing
2 ug/lane SDS-PAGE Stained with Coomassie Blue
Storage Buffer
In 50 mM Hepes, 100 mM NaCl, pH 7.5. (5 mM DTT, 20% glycerol)
Storage Instruction
Store at -80°C.
Aliquot to avoid repeated freezing and thawingNote
Result of activity analysis
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Applications
Functional Study
SDS-PAGE
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Gene Info — PRKD3
Entrez GeneID
23683Protein Accession#
AB015982.2Gene Name
PRKD3
Gene Alias
EPK2, PKC-NU, PKD3, PRKCN, nPKC-NU
Gene Description
protein kinase D3
Omim ID
607077Gene Ontology
HyperlinkGene Summary
Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role. The protein encoded by this gene is one of the PKC family members. This kinase can be activated rapidly by the agonists of G protein-coupled receptors. It resides in both cytoplasm and nucleus, and its nuclear accumulation is found to be dramatically enhanced in response to its activation. This kinase can also be activated after B-cell antigen receptor (BCR) engagement, which requires intact phopholipase C gamma and the involvement of other PKC family members. [provided by RefSeq
Other Designations
OTTHUMP00000126953|protein kinase C, nu|protein kinase EPK2|protein-serine/threonine kinase
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