PRKCD (Human) Recombinant Protein
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Result of activity analysis
Result of activity analysis
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Specification
Product Description
Human PRKCD (NM_006254.3, 1 a.a. - 675 a.a.) full-length recombinant protein with GST-His tag expressed in Sf9 cells.
Host
insect
Theoretical MW (kDa)
111.765
Form
Liquid
Preparation Method
Insect cell (Sf9) expression system
Purification
One-step affinity purification using GSH agarose
Concentration
0.162 ug/uL
Activity
242 pmol/ug x min
Quality Control Testing
2 ug/lane SDS-PAGE Stained with Coomassie Blue
Storage Buffer
In 50 mM Tris-HCl, 100 mM NaCl, pH 8.0. (5 mM DTT, 15 mM reduced glutathione, 20% glycerol)
Storage Instruction
Store at -80°C.
Aliquot to avoid repeated freezing and thawingNote
Result of activity analysis
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Applications
Functional Study
SDS-PAGE
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Gene Info — PRKCD
Entrez GeneID
5580Protein Accession#
NM_006254.3Gene Name
PRKCD
Gene Alias
MAY1, MGC49908, PKCD, nPKC-delta
Gene Description
protein kinase C, delta
Omim ID
176977Gene Ontology
HyperlinkGene Summary
Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play distinct roles in cells. The protein encoded by this gene is one of the PKC family members. Studies both in human and mice demonstrate that this kinase is involved in B cell signaling and in the regulation of growth, apoptosis, and differentiation of a variety of cell types. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq
Other Designations
protein kinase C delta VIII
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Interactome
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Pathway
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Disease
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