CDK4/CCND3 (Human) Recombinant Protein
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Specification
Product Description
Human CDK4 (NM_000075, 4 a.a. - 303 a.a.) and CCND3 (NM_001760, 1 a.a. - 292 a.a.) Recombinant protein with His tag expressed in Sf9 cells.
Host
insect
Theoretical MW (kDa)
CDK4: 59.700 , CCND3
Form
Liquid
Preparation Method
Insect cell (Sf9) expression system
Purification
One-step affinity purification using GSH-agarose
Concentration
0.337 ug/uL
Activity
75 pmol/ug x min
Quality Control Testing
2 ug/lane SDS-PAGE Stained with Coomassie Blue
Storage Buffer
In 50 mM Tris-HCl, 100 mM NaCl, pH 8.0. (5 mM DTT, 4 mM reduced glutathione, 20% glycerol)
Storage Instruction
Store at -80°C.
Aliquot to avoid repeated freezing and thawingNote
Result of activity analysis
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Applications
Functional Study
SDS-PAGE
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Gene Info — CCND3
Entrez GeneID
896Protein Accession#
NM_000075 (Gene ID : 1019);NM_001760 (Gene ID : 896)Gene Name
CCND3
Gene Alias
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Gene Description
cyclin D3
Omim ID
123834Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activtiy is required for cell cycle G1/S transition. This protein has been shown to interact with and be involved in the phosphorylation of tumor suppressor protein Rb. The CDK4 activity associated with this cyclin was reported to be necessary for cell cycle progression through G2 phase into mitosis after UV radiation. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
D3-type cyclin|G1/S-specific cyclin D3|OTTHUMP00000016390
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Gene Info — CDK4
Entrez GeneID
1019Protein Accession#
NM_000075 (Gene ID : 1019);NM_001760 (Gene ID : 896)Gene Name
CDK4
Gene Alias
CMM3, MGC14458, PSK-J3
Gene Description
cyclin-dependent kinase 4
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is highly similar to the gene products of S. cerevisiae cdc28 and S. pombe cdc2. It is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression. The activity of this kinase is restricted to the G1-S phase, which is controlled by the regulatory subunits D-type cyclins and CDK inhibitor p16(INK4a). This kinase was shown to be responsible for the phosphorylation of retinoblastoma gene product (Rb). Mutations in this gene as well as in its related proteins including D-type cyclins, p16(INK4a) and Rb were all found to be associated with tumorigenesis of a variety of cancers. Multiple polyadenylation sites of this gene have been reported. [provided by RefSeq
Other Designations
cell division kinase 4|melanoma cutaneous malignant, 3
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Interactome
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Pathway
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Disease
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