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CDK4/CCND1 (Human) Recombinant ProteinBioActive

  • Catalog # : P4657
  • Visit Frequency :
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  • Specification
  • Product Description:
  • Human CDK4 (NM_001786, 4 a.a. - 303 a.a.) and CCND1 (NM_053056, 4 a.a. - 295 a.a.) recombinanat protein with GST tag expressed in Sf9 cells.
  • Host:
  • Insect
  • Theoretical MW (kDa):
  • CDK4: 59.700 , CCND1
  • Form:
  • Liquid
  • Preparation Method:
  • Insect cell (Sf9) expression system
  • Purification:
  • GST affinity chromatography
  • Concentration:
  • 0.314 ug/uL
  • Activity:
  • 99 pmol/ug x min
  • Quality Control Testing:
  • 2 ug/lane SDS-PAGE Stained with Coomassie Blue

    QC Testing of P4657
  • Storage Buffer:
  • In 50 mM Hepes, 100 mM NaCl, pH 7.5. (5 mM DTT, 4 mM reduced glutathione, 20% glycerol)
  • Storage Instruction:
  • Store at -80°C.
    Aliquot to avoid repeated freezing and thawing
  • Note:
  • Result of activity analysis

  • Applications
  • Functional Study
  • SDS-PAGE
  • Application Image
  • Functional Study
  • SDS-PAGE
  • Gene Information
  • Entrez GeneID:
  • 1019
  • Protein Accession#:
  • NM_000075 (Gene ID : 1019);NM_053056 (Gene ID : 595)
  • Gene Name:
  • CDK4
  • Gene Alias:
  • CMM3,MGC14458,PSK-J3
  • Gene Description:
  • cyclin-dependent kinase 4
  • Gene Summary:
  • The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is highly similar to the gene products of S. cerevisiae cdc28 and S. pombe cdc2. It is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression. The activity of this kinase is restricted to the G1-S phase, which is controlled by the regulatory subunits D-type cyclins and CDK inhibitor p16(INK4a). This kinase was shown to be responsible for the phosphorylation of retinoblastoma gene product (Rb). Mutations in this gene as well as in its related proteins including D-type cyclins, p16(INK4a) and Rb were all found to be associated with tumorigenesis of a variety of cancers. Multiple polyadenylation sites of this gene have been reported. [provided by RefSeq
  • Other Designations:
  • cell division kinase 4,melanoma cutaneous malignant, 3
  • Gene Information
  • Entrez GeneID:
  • 595
  • Protein Accession#:
  • NM_000075 (Gene ID : 1019);NM_053056 (Gene ID : 595)
  • Gene Name:
  • CCND1
  • Gene Alias:
  • BCL1,D11S287E,PRAD1,U21B31
  • Gene Description:
  • cyclin D1
  • Gene Summary:
  • The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of tumors and may contribute to tumorigenesis. [provided by RefSeq
  • Other Designations:
  • B-cell CLL/lymphoma 1,G1/S-specific cyclin D1
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