AURKB (Human) Recombinant Protein
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Result of activity analysis
Result of activity analysis
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Specification
Product Description
Human AURKB (NM_004217, 2 a.a. - 344 a.a.) partial recombinant protein with GST-His tag expressed in Sf9 cells.
Host
insect
Theoretical MW (kDa)
70.07299999999979
Form
Liquid
Preparation Method
Insect cell (Sf9) expression system
Purification
GST Affinity Chromatography
Concentration
0.196 ug/uL
Activity
37 pmol/ug x min
Quality Control Testing
2 ug/lane SDS-PAGE Stained with Coomassie Blue
Storage Buffer
In 50 mM Hepes, 100 mM NaCl, pH 7.5. (5 mM DTT, 4 mM reduced glutathione, 20% glycerol)
Storage Instruction
Store at -80°C.
Aliquot to avoid repeated freezing and thawingNote
Result of activity analysis
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Applications
Functional Study
SDS-PAGE
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Gene Info — AURKB
Entrez GeneID
9212Protein Accession#
NM_004217Gene Name
AURKB
Gene Alias
AIK2, AIM-1, AIM1, ARK2, AurB, IPL1, STK12, STK5
Gene Description
aurora kinase B
Omim ID
604970Gene Ontology
HyperlinkGene Summary
Chromosomal segregation during mitosis as well as meiosis is regulated by kinases and phosphatases. The Aurora kinases associate with microtubules during chromosome movement and segregation. Aurora kinase B localizes to microtubules near kinetochores, specifically to the specialized microtubules called K-fibers, and Aurora kinase A (MIM 603072) localizes to centrosomes (Lampson et al., 2004 [PubMed 14767480]).[supplied by OMIM
Other Designations
aurora-1|aurora-B|serine/threonine kinase 12
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