Il31 (Mouse) Recombinant Protein
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Lane 1: non-reducing conditions
Lane 2: reducing conditions
Result of activity analysis
Result of activity analysis
Human PBMCs were cultured with 0 to 1000 ng/mL mouse Il31. Human IL-8 production was measured after 48 hours and the linear portion of the curve was us used to calculate the ED50.
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Specification
Product Description
Mouse Il31 (Q6EAL8) recombinant protein expressed in Escherichia coli.
Sequence
MTCSLSFGAPISKEDLRTTIDLLKQESQDLYNNYSIKQASGMSADESIQLPCFSLDREALTNISVIIAHLEKVKVLSENTVDTSWVIRWLTNISCFNPLNLNISVPGNTDESYDCKVFVLTVLKQFSNCMAELQAKDNTTC
Host
Escherichia coli
Theoretical MW (kDa)
15.7
Form
Lyophilized
Preparation Method
Escherichia coli expression system
Endotoxin Level
< 0.1 EU/ug
Activity
The activity is determined by the dose dependent production of IL-8 by human PBMCs. The expected ED50 for this effect is 16-24 pg/mL.
Quality Control Testing
1 ug/lane in 4-20% Tris-Glycine gel Stained with Coomassie Blue
Lane 1: non-reducing conditions
Lane 2: reducing conditionsStorage Buffer
Lyophilized with 10 mM Na2PO4, pH 7.5.
Storage Instruction
Store at -20°C on dry atmosphere.
After reconstitution with sterilized water, store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of activity analysis
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Applications
Functional Study
SDS-PAGE
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Gene Info — Il31
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