Cxcl12 (Beta) (Mouse) Recombinant Protein
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Lane 1: non-reducing conditions
Lane 2: reducing conditions
Result of activity analysis
Result of activity analysis
Triplicate samples of primary human neutrophils from three donors were allowed to migrate to mouse Cxcl12 (beta) (10, 100 and 1000 ng/mL). After 30 minutes, cells that migrated were counted using a luminescent substrate and displayed on the bar graph above.
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Specification
Product Description
Mouse Cxcl12 (beta) (P40224-2) recombinant protein expressed in Escherichia coli.
Sequence
KPVSLSYRCPCRFFESHIARANVKHLKILNTPNCALQIVARLKNNNRQVCIDPKLKWIQEYLEKALNKRLKM
Host
Escherichia coli
Theoretical MW (kDa)
8.5
Form
Lyophilized
Preparation Method
Escherichia coli expression system
Endotoxin Level
< 0.1 EU/ug
Activity
The activity is determined by the ability to chemoattract human peripheral T cells and is detectable at 5-100 ng/mL.
Quality Control Testing
1 ug/lane in 4-20% Tris-Glycine gel Stained with Coomassie Blue
Lane 1: non-reducing conditions
Lane 2: reducing conditionsStorage Buffer
No additive
Storage Instruction
Store at -20°C on dry atmosphere.
After reconstitution with sterilized water, store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing.Note
Result of activity analysis
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Applications
Functional Study
SDS-PAGE
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Gene Info — Cxcl12
Entrez GeneID
20315Protein Accession#
P40224-2Gene Name
Cxcl12
Gene Alias
AI174028, PBSF, PBSF/SDF-1, SDF-1, Scyb12, Sdf1, Sdf1a, Sdf1b, TLSF, TLSF-a, TLSF-b, TPAR1
Gene Description
chemokine (C-X-C motif) ligand 12
Gene Ontology
HyperlinkOther Designations
OTTMUSP00000026112|OTTMUSP00000026113|OTTMUSP00000026114|pre-B-cell growth-stimulating factor|stromal cell derived factor 1
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