Ccl8 (Mouse) Recombinant Protein
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Lane 1: non-reducing conditions
Lane 2: reducing conditions
Result of activity analysis
Result of activity analysis
Human THP-1 cells were allowed to migrate to mouse Ccl8 at (0, 0.1, 1, and 10ng/mL). After 45 minutes, cells that migrated were counted using a luminescent substrate and displayed on the bar graph above.
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Specification
Product Description
Mouse Ccl8 (Q9Z121) recombinant protein expressed in Escherichia coli.
Sequence
GPDKAPVTCCFHVLKLKIPLRVLKSYERINNIQCPMEAVVFQTKQGMSLCVDPTQKWVSEYMEILDQKSQILQP
Host
Escherichia coli
Theoretical MW (kDa)
8.5
Form
Lyophilized
Preparation Method
Escherichia coli expression system
Endotoxin Level
< 0.1 EU/ug
Activity
The activity is determined by the ability to chemoattract THP-1 cells and is detectable starting at 0.1 ng/mL.
Quality Control Testing
1 ug/lane in 4-20% Tris-Glycine gel Stained with Coomassie Blue
Lane 1: non-reducing conditions
Lane 2: reducing conditionsStorage Buffer
No additive
Storage Instruction
Store at -20°C on dry atmosphere.
After reconstitution with sterilized water, store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of activity analysis
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Applications
Functional Study
SDS-PAGE
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Gene Info — Ccl8
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