Shh (Mouse) Recombinant Protein
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Result of activity analysis
Result of activity analysis
Serial dilutions of mouse Shh, starting at 5 ug/mL, were added to with CCL-226 cells in the presence of 1 uM Retinoic Acid. Alkaline phosphatase was measured and the linear portion of the curve was us used to calculate the ED50.
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Specification
Product Description
Mouse Shh (Q15465) recombinant protein expressed in Escherichia coli.
Sequence
MIIGPGRGFGKRRHPKKLTPLAYKQFIPNVAEKTLGASGRYEGKITRNSERFKELTPNYNPDIIFKDEENTGADRLMTQRCKDKLNALAISVMNQWPGVKLRVTEGWDEDGHHSEESLHYEGRAVDITTSDRDRSKYGMLARLAVEAGFDWVYYESKAHIHCSVKAENSVAAKSGG
Host
Escherichia coli
Theoretical MW (kDa)
19.8
Form
Lyophilized
Preparation Method
Escherichia coli expression system
Endotoxin Level
< 0.1 EU/ug
Activity
The activity is determined by the dose-dependent increase of alkaline phosphatase activity in C3H/10T1/2 fibroblasts (CCL-226). The expected ED50 for this effect is 0.48-0.72 ug/mL.
Storage Buffer
Lyophilized with 10 mM Na2PO4, pH 7.5.
Storage Instruction
Store at -20°C on dry atmosphere.
After reconstitution with sterilized water, store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of activity analysis
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Applications
Functional Study
SDS-PAGE
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Gene Info — Shh
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