The activity is determined by the dose-dependent inhibition of IL-17A expression from mouse CD4+ splenocytes stimulated withTGF-beta and IL-6. 50 ng/mL of mouse p28 inhibits greater than 25% of IL-17A expression.
Quality Control Testing:
1 ug/lane in 4-20% Tris-Glycine gel Stained with Coomassie Blue
Lane 1: non-reducing conditions Lane 2: reducing conditions
Lyophilized with Na2PO4, pH 7.5.
Store at -20°C on dry atmosphere. After reconstitution with sterilized water, store at -20°C. Aliquot to avoid repeated freezing and thawing.