Il16 (Mouse) Recombinant Protein
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Lane 1: non-reducing conditions
Lane 2: reducing conditions
Result of activity analysis
Result of activity analysis
Human T cells were allowed to migrate to mouse Il16 at (0, 5, 20, 100 and 500 ng/mL). After 4 hours, cells that migrated were counted using a luminescent substrate and displayed on the bar graph above.
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Specification
Product Description
Mouse Il16 (O54824) recombinant protein expressed in Escherichia coli.
Sequence
MHDLNSSTDSAASASAASDISVESKEATVCTVTLEKTSAGLGFSLEGGKGSLHGDKPLTINRIFKGDRTGEMVQPGDEILQLAGTAVQGLTRFEAWNVIKALPDGPVTIVIRRTSLQCKQTTASADS
Host
Escherichia coli
Theoretical MW (kDa)
13.2
Form
Lyophilized
Preparation Method
Escherichia coli expression system
Endotoxin Level
< 0.1 EU/ug
Activity
The activity is determined by the dose-dependent chemoattraction of primary human T cells and is detectable starting at 5 ng/mL.
Quality Control Testing
1 ug/lane in 4-20% Tris-Glycine gel Stained with Coomassie Blue
Lane 1: non-reducing conditions
Lane 2: reducing conditionsStorage Buffer
Lyophilized 10 mM Na2PO4, pH 7.5.
Storage Instruction
Store at -20°C on dry atmosphere.
After reconstitution with sterilized water, store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of activity analysis
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Applications
Functional Study
SDS-PAGE
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Gene Info — Il16
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