Vegfa (Mouse) Recombinant Protein
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Lane 1: non-reducing conditions
Lane 2: reducing conditions
Result of activity analysis
Result of activity analysis
Serial dilutions of murine Vegfa, starting at 100 ng/mL, were added to HUVECs. After 92 hours, cell proliferation was measured and the linear portion of the curve was us used to calculate the ED50.
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Specification
Product Description
Mouse Vegfa (165 amino acids) (AAA40547) recombinant protein expressed in Escherichia coli.
Sequence
MAPTTEGEQKSHEVIKFMDVYQRSYCRPIETLVDIFQEYPDEIEYIFKPSCVPLMRCAGCCNDEALECVPTSESNITMQIMRIKPHQSQHIGEMSFLQHSRCECRPKKDRTKPENHCEPCSERRKHLFVQDPQTCKCSCKNTDSRCKARQLELNERTCRCDKPRR
Host
Escherichia coli
Theoretical MW (kDa)
39
Form
Lyophilized
Preparation Method
Escherichia coli expression system
Endotoxin Level
< 0.1 EU/ug
Activity
The activity is determined by the dose-dependent proliferation of human umbilical vein endothelial cells (HUVEC) using a concentration range of 1.9-2.8 ng/mL.
Quality Control Testing
1 ug/lane in 4-20% Tris-Glycine gel Stained with Coomassie Blue
Lane 1: non-reducing conditions
Lane 2: reducing conditionsStorage Buffer
No additive
Storage Instruction
Store at -20°C on dry atmosphere.
After reconstitution with sterilized water, store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of activity analysis
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Applications
Functional Study
SDS-PAGE
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Gene Info — Vegfa
Entrez GeneID
22339Protein Accession#
AAA40547Gene Name
Vegfa
Gene Alias
Vegf, Vegf-a, Vegf120, Vegf164, Vegf188, Vpf
Gene Description
vascular endothelial growth factor A
Gene Ontology
HyperlinkOther Designations
OTTMUSP00000017463|OTTMUSP00000017464|OTTMUSP00000022243|vascular permeability factor
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