The activity is determined by the dose-dependent proliferation of human umbilical vein endothelial cells (HUVEC) using a concentration range of 1.9-2.8 ng/mL.
Quality Control Testing:
1 ug/lane in 4-20% Tris-Glycine gel Stained with Coomassie Blue
Lane 1: non-reducing conditions Lane 2: reducing conditions
Storage Buffer:
No additive
Storage Instruction:
Store at -20°C on dry atmosphere. After reconstitution with sterilized water, store at -20°C. Aliquot to avoid repeated freezing and thawing.
Note:
Result of activity analysis
Serial dilutions of murine Vegfa, starting at 100 ng/mL, were added to HUVECs. After 92 hours, cell proliferation was measured and the linear portion of the curve was us used to calculate the ED50.