The activity is determined by the dose-dependent proliferation of murine NR6R 3T3 cells. The expected ED50 for this effect is 0.9-1.4 ng/mL.
Quality Control Testing:
1 ug/lane in 4-20% Tris-Glycine gel Stained with Coomassie Blue
Lane 1: non-reducing conditions Lane 2: reducing conditions
Storage Buffer:
Lyophilized with 10 mM Na2PO4, 75 mM (NH4)2SO4, pH 7.5.
Storage Instruction:
Store at -20°C on dry atmosphere. After reconstitution with sterilized water, store at -20°C. Aliquot to avoid repeated freezing and thawing.
Note:
Result of activity analysis
3T3 cells were cultured with 0 to 100 ng/mL mouse Fgf9. Cell proliferation was measured after 42 hours and the linear portion of the curve was us used to calculate the ED50.