The activity is determined by the proliferation of TF-1 cells. The expected ED50 for this effect is 0.3-0.4 ng/mL.
Quality Control Testing:
1 ug/lane in 4-20% Tris-Glycine gel Stained with Coomassie Blue
Lane 1: non-reducing conditions Lane 2: reducing conditions
Storage Buffer:
No additive
Storage Instruction:
Store at -20°C on dry atmosphere. After reconstitution with sterilized water, store at -20°C. Aliquot to avoid repeated freezing and thawing.
Note:
Result of activity analysis
Serial dilutions of mouse Ngf, starting at 100 ng/mL, were added to TF-1 cells growing in GM-SCF free media. Cell proliferation was measure after 63 hours and the linear portion of the curve was us used to calculate the ED50.