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Last updated: 2017/7/23

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Ngf (Mouse) Recombinant ProteinBioActive

  • Catalog # : P4591
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  • Specification
  • Product Description:
  • Mouse Ngf (Q6LDU8) recombinant protein expressed in Escherichia coli.
  • Sequence:
  • MSSTHPVFHMGEFSVCDSVSVWVGDKTTATDIKGKEVTVLAEVNINNSVFRQYFFETKCRASNPVESGCRGIDSKHWNSYCTTTHTFVKALTTDEKQAAWRFIRIDTACVCVLSRKATRRG
  • Host:
  • Escherichia coli
  • Theoretical MW (kDa):
  • 13.5
  • Form:
  • Lyophilized
  • Preparation Method:
  • Escherichia coli expression system
  • Endotoxin Level:
  • < 0.1 EU/ug
  • Activity:
  • The activity is determined by the proliferation of TF-1 cells. The expected ED50 for this effect is 0.3-0.4 ng/mL.
  • Quality Control Testing:
  • 1 ug/lane in 4-20% Tris-Glycine gel Stained with Coomassie Blue

    QC Testing of P4591
    Lane 1: non-reducing conditions
    Lane 2: reducing conditions
  • Storage Buffer:
  • No additive
  • Storage Instruction:
  • Store at -20°C on dry atmosphere.
    After reconstitution with sterilized water, store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • Result of activity analysis

  • Serial dilutions of mouse Ngf, starting at 100 ng/mL, were added to TF-1 cells growing in GM-SCF free media. Cell proliferation was measure after 63 hours and the linear portion of the curve was us used to calculate the ED50.


  • Applications
  • Functional Study
  • SDS-PAGE
  • Application Image
  • Functional Study
  • SDS-PAGE
  • Gene Information
  • Gene Name:
  • Ngf
  • Gene Alias:
  • Ngfb
  • Gene Description:
  • nerve growth factor
  • Other Designations:
  • nerve growth factor, beta
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