The activity is determined by the dose-dependent induction of HUVECproliferation. The expected ED50 for this effect is 1.1-1.7 ?g/mL.
Quality Control Testing:
1 ug/lane in 4-20% Tris-Glycine gel Stained with Coomassie Blue
Lane 1: non-reducing conditions Lane 2: reducing conditions
Storage Buffer:
Lyophilized with 10 mM sodium acetate, pH 6.0.
Storage Instruction:
Store at -20°C on dry atmosphere. After reconstitution with 5 mM sodium acetate, store at -20°C. Aliquot to avoid repeated freezing and thawing.
Note:
Result of activity analysis
Serial dilutions of human CTGF, starting at 10 ug/mL, were added to HUVECs cultured without EGF. Cell proliferation was measured after 87 hours and the linear portion of the curve was us used to calculate the ED50.